Q-PHASE: Quantitative, label-free imaging cytometry

Home > Products > Tescan

Q-PHASE a valuable research tool for biological and biotechnical applications such as testing reactions of cells to a speci c treatment – even with scattering non-transparent substances, monitoring cell’s life cycle including mitosis, distinguishing between di erent forms of cell deaths, analyzing cell growth, motility or morphology changes, imaging cells in extracellular matrices.

Features

Simple Automated Image segmentation and processing – comparable to fluorescence data processing
No image artifacts such as halo effect (as opposed to techniques based on Zernike phase contrast illumination)
Enables very precise detection of cell boundaries
Label-free – no staining is needed, simple sample preparation, observation of live cells in their native environment, no photobleaching problems
Low phototoxicity – low light power density (107 × lower than fluorescence microscopy) allows long-term observations (for days)
Coherence-gating effect – Q-PHASE special feature enabling to observe samples even in scattering media (phospholipid emulsions, extracellular matrices, etc.)
Multimodality – fully integrated fluorescence module, simulated DIC and brightfield which enables automatic multimodal imaging of the sample
High-quality QPI – unique Q-PHASE’s optical setup allows using incoherent illumination which provides extraordinary imaging quality without any compromises
Strong suppression of coherent noise (speckles) & parasitic interferences (as opposed to laser-based approaches)
Lateral resolution of conventional microscopes (up to 2× better when compared to common laser-based approaches or pinhole spatial filtering based techniques)
Fast acquisition – the use of off-axis holographic approach makes Q-PHASE a single-shot instrument, thus enabling imaging of very fast cell dynamics
Full motorization – focusing, sample stage, objective exchange, fluorescence filters
Automated multidimensional acquisition – time-lapse, channel, position, Z-stack
Quantitative – phase values can be recalculated e.g. to cell dry-mass density (pg/μm2) or direct topography with nanometer sensitivity (usually non-biological samples with homogeneous refractive index distribution)
High phase detection sensitivity – enables to detect even the smallest changes in axial direction, very sensitive detection of morphology or position changes

Applications

For Support Services

Phone: (91-22) 2500 2631 / 2500 2632

Mail: mumbai@advsci.spsipl.co.in / sales@advsci.spsipl.co.in / service@advsci.spsipl.co.in

Q-PHASE: Quantitative, label-free imaging cytometry

Features

Applications

For Support Services

About Us

Useful Links

Our Principals

Contact Us

Advance Scientific Equipment Pvt. Ltd.